At Xoligo Biologics, we recognize that nucleic acid therapeutics often rely on microbial systems as their foundation. From plasmid DNA backbones to enzymes and capsid proteins, microbial fermentation underpins RNA and oligonucleotide development. Our fermentation services are designed to provide the upstream strength and flexibility needed to support cutting-edge biopharma programs.
We offer a platform that combines strain engineering, scalable fermentation, and rigorous quality control, ensuring reliable microbial products for research, clinical, and therapeutic applications.
Plasmid DNA Fermentation
Plasmids remain the backbone of RNA production and gene editing tools.
- E. coli–Based Systems – Optimized strains for high-yield plasmid generation.
- Scalable Production – From 1 L bench-scale to multi-hundred-liter bioreactors.
- High Purity – Endotoxin-free plasmid prep, suitable for RNA synthesis and preclinical use.
- Custom Backbones – Promoters, origins of replication, and regulatory elements tailored to client needs.
Enzyme Production
The enzymes that power nucleic acid workflows are often derived from microbial hosts.
- Polymerases & Ligases – High-activity enzymes produced via fermentation.
- Custom Enzymes – Specialty proteins engineered for client-specific processes.
- Expression Optimization – Codon usage and fermentation tuning for maximal yield.
- Purification & QC – Multi-step purification with full activity characterization.
Protein & Capsid Expression
Microbes remain powerful platforms for structural protein production.
- Capsid Proteins – Bacterial systems for phage and viral vector components.
- Exosomes & Nanoparticles – Microbial expression of advanced scaffolds.
- Vaccines & Carriers – Fermentation systems for recombinant protein vaccines.
- Analytical Support – Identity, purity, and functional validation built into every run.
Specialty Fermentation
We go beyond standard plasmids and proteins, offering fermentation for specialized applications.
- Diagnostic Enzymes – Scalable production for molecular diagnostics.
- Probiotics & Microbial Biologics – Tailored fermentations for live-cell products.
- Phage & Engineered Strains – Support for bacteriophage or synthetic biology programs.
- Custom Projects – Designed in collaboration with clients for unique microbial needs.
Quality & Flexibility
Our microbial platform is built to complement nucleic acid development, offering upstream solutions that fit seamlessly with downstream RNA and oligonucleotide workflows.
- Fermentation Control – Precise monitoring of pH, DO, and feed strategies.
- Purification Systems – Chromatography, TFF, and endotoxin removal.
- Scalable Batches – Discovery-scale through pilot-scale lots.
- Regulatory Readiness – Documentation aligned with preclinical and GMP expectations.
The Xoligo Difference
By integrating microbial fermentation with our nucleic acid expertise, we provide clients with a complete development path. Whether you need plasmid DNA for RNA synthesis, enzymes for custom workflows, or microbial expression of novel biologics, Xoligo delivers precision, scalability, and trust in every project.